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AG Scientific/Ulex europaeus (Gorse) lectin (UEA I) - Separopore®/U-2589/3 ml
Applications:
Ulexeuropaeus agglutininI(UEA)isaglycoproteinwithamolecularweightof63kDa,althoughmultimericaggregateshavebeenreported.UEAIhastwosubunits,oneofabout31kDaandanotherof32kDa.UEAIbindstomanyglycoproteinsandglycolipidscontaining α -linkedfucoseresidues,suchasABObloodgroupglycoconjugates.ThislectinpreferentiallybindsbloodgroupOcellsandhasbeenusedtodeterminesecretorstatus.IthasbeenestablishedasanexcellentMarkerforhumanendothelialcells.
UEAisolatedfrom Ulexeuropaeus (Furzegorse)seedsiscoupledwithSeparopore®4B-CL.Heatstable,cross-linked4%agarosebeadswithamolecularweightexclusionlimitofabout2x107 areusedasthesolid-phasematrixtowhichthelectiniscovalentlybound.Theattachmentofthelectintothesolidphaseiscontrolled sothattheactivityofthelectinpreservedandconformationalchangesoftheboundlectinareminimized.
TechnicalSpecifications:
- Ligand: Ulexeuropaeus
- Matrix:Separopore4B-CL(crosslinkedagarose,4%)
- Particlesizerange:52-165µm
- Matrixactivation:Cyanogenbromide
- Matrixattachment:Amino
- Liganddensity:2–4mgUEA/mLdrainedgel
- Bindingcapacity:3mgfucosylglycoprotein/mldrainedgel
- Inhibiting/ElutingSugar:50mM-100mML-fucose
- CarbohydrateforElution:.2MFucose
- BloodGroupSpecificity:H-type2
- Form:50:50LiquidSUSPension,PBS(1x)pH7.4w/0.01%Thimerosal
- pHstABIlity:4–9
AdditionalInformation
| Product# | U-2589 |
|---|---|
| Appearance | LiquidSuspension |
| StorageTemp | 4°C |
