LeupeptinisareversIBLecompetitiveinhibitorofserineandthiolproteases.Ithasbeenreportedtoinhibitcalpain,cathepsinB,cathepsinsHandLandtrypsin.Atypicalworkingconcentrationsisintherangeof10to100µM.Leupeptinappearstobeequallyeffectiveinanysaltform,adjustingforequivalentpeptidecontent.Thehemisulfatesaltswerethefirsttobecommerciallyavailable.Ofthethreesalts,thehydrochlorideistheleastinvasiveforminBIOLOGicalsettings.Noproblemorpreferenceforthetrifluoroacetate(TFA)formhasbeennotes;TFAisvolatile,socouldpossiblyberemovedbylyophilization.
Microbiallyproducedleupeptininhibitorwasfirstisolatedasamixtureoftwoverysimilarforms:acetyl-LeuLeu-Arg-alandpropionyl-Leu-Leu-Arg-al.Althoughthepropionylleupeptinisactiveasaninhibitortheacetylformismorecommonlyusedandisavailableinseveraldifferentforms.
Leupeptin,becauseofitsaldehydegroup,mayactasareducingagentandthereforeinterfereinproteindeterminationssuchasLowryand,toalesserextent,BradfordTheactivityofleupeptinsandrelatedanalogshasbeenstudied.
Leupeptinstock(20mg/ml):LeupeptininMethanol.Prepareastockof20mg/mlinmethanol,Storeat-20°C.
AdditionalInformation
| Synonyms | Acetyl-Leu-Leu-Arg-altrifluoroacetatesalt |
|---|---|
| Product# | L-3315 |
| CAS# | 147385-61-3 |
| Formula | C20H38N6O4⋅C2HF3O2 |
| MW | 540.6 |
| Appearance | WhiteSolid |
| Purity | >95% |
| Solubility | 50mg/mlinwater |
| StorageTemp | -20°C |
