Applications:
Lensculinaris agglutinin(LCA)hasamolecularweightofapproximately49kDaandiscomposedoffoursubunits-twoofabout17kDaandtwoof8kDa.TheisoelectricpointofLCAisapproximatelypH8.5.LCArecognizessequencescontaining a-linkedmannoseresidues.Likeothermannosespecificlectins,divalentcationssuchascalciumandmanganesearerequiredforsugarbindingactivity.
LCAhasbeenemployedtoseparatelymphocytepopulations,asapotentT-cellmitogen,andasoneofthemosteffectiveagentsinpreventingskinallograftrejectioninmodelsystems.LCAisalsousedtopurifynumerousglycoproteins(includingimmunoglobulins,histocompatibilityantigens, α2-macroglobulin,etc.)aswellastofractionateglycopeptidesfromavarietyofglycoproteins.
LensCulinaris Agglutininisolatedfrom Lensculinaris (lentil)seeds is coupledtoheatstable,cross-linked4%agarosebeadswithamolecularweightexclusionlimitofabout2x107 usedasthesolid-phasematrixtowhichthelectiniscovalentlybound.Theattachmentofthelectintothesolidphaseiscarefullycontrolledinordertopreservetheactivityofthelectinaswellastominimizeconformationalchangesoftheboundlectin.
TechnicalSpecifications
- Ligand: Lensculinaris agglutinin(LCA)
- Matrix:4B-CL(crosslinkedagarose,4%)
- Particlesizerange:52–165µm
- Exclusionlimit:Approximately2x107
- Matrixactivation:Cyanogenbromide
- Matrixattachment:Amino
- Liganddensity: 2–4mgLCA/mldrainedgel
- BindingCapacity: 3 mgMannosylglycoprotein/mldrainedgel
- Inhibiting/ElutingSugar:a-Methylglucoside+a-Methylmannoside
- CarbohydrateforElution: 0.1M α-MethylMannopyranosideor0.1MD-MannoseinBuffer
- BloodGroupSpecificity:Notavailable
- Form:50:50LiquidSUSPension,PBS(1x)pH7.4w/0.01%Thimerosal
- pHstABIlity:4–9
AdditionalInformation
| Product# | L-2637 |
|---|---|
| Appearance | LiquidSuspension |
| StorageTemp | 4°C |
