Applications:
Reducedglutathioneiscovalentlylinked 4Bforuseinaffinitypurificationofglutathione-S-transferase(GST)andGST-fusedproteins.GlutathioneSuperFlow(SF)isusedforproteinpurificationbybatch/columnandscale-upprocedures.GlutathioneSuperFlow(SF)hasanexellentproteinbindingcapacity.Thegoodflowpropertiesofthe92μm(averagesize)highlycrosslinkedmatrixmakesanexcellentchoiceforproteinpurificationbycolumnpurificationorbatchprocessforscale-up.
Thisproductprovidesaonesteppurificationmethodandpermitsrapid,mildandhighlyselectivepurificationsofproteinscontainingglutathionebindingsequences. Glutathione-Separoporeispreparedbycovalentlycouplingglutathionetoepoxy-activated4%crosslinkedagarosebeadstoformastablethioetherlinkage.Thecouplingwasoptimizedtogiveahighbindingcapacityof5mgormoreofGlutathione-S-transferase(GST)permlofwetgel. BoundGST–fusionproteinsareeasilydisplacedfromtheresinbyelutionwithbufferscontainingreducedglutathione.Mildelutionconditionspreserveproteinantigenicityandfunction.
Technicalspecifications:
- Ligand:Reducedglutathione(GSH)
- Matrix:4B-CL(highlycrosslinkedagarosebeads,4%)
- Particlesizerange:52–165µm
- Molecularweightrange:6x104 –2x107
- Matrixactivation:Epoxy
- Matrixspacer:12atoms
- Liganddensity: 5–10mgGST/mldrainedgel
- Bindingcapacity:5–10mgrecombinantGST-taggedprotein/mldrainedgel
- pHstABIlity:3–12
- Storage:2°C–8°C
- Suppliedas50%slurryin20%ethanol
AdditionalInformation
| Product# | G-2625 |
|---|---|
| Appearance | LiquidSUSPension |
| StorageTemp | 4°C |
