Applications:
Erythrinacristagalli (coraltree)lectinisa54kDaglycoproteinconsistingoftwodifferentsubunitsofapproximately28and26kDa.Ithasaspecificitytowardgalactoseresiduesandappearstohavethehighestbindingactivitytowardgalactosyl(β-1,4)N-acetylglucosamine.
Erythrinacristagalli Lectin(ECL) isolatedfrom Erythrinacristagalli (coraltree)seeds coupledwith 4B-CL ispreparedwith affinity-purifiedlectin.Theheatstable,cross-linked4%agarosebeadswithamolecularweightexclusionlimitofabout2x107 areusedasthesolid-phasematrixtowhichthelectiniscovalentlybound.Theattachmentofthelectintothesolidphaseiscarefullycontrolledinordertopreservetheactivityofthelectinaswellastominimizeconformationalchangesoftheboundlectin.
Erythrinacristagalli Lectin(ECL)-4B-CL hasbeenreportedtobeusefulfortheisolationofhumannaturalkiller(NK)cellsusinganegativeselectionpanningtechnique.HumanNKcellsappeartolackaccessIBLesurfacecarbohydratestructuresrequiredforbindingECLand,unlikeothermononuclearcells,donotadheretoECL-coatedculturedishesorECLcoupledagarosebeads.Sincethisprocedureinvolvesanegativeselectionpanningtechnique,ahighrecoveryofviableNKcellscanbeobtained.Theadherentcellscanalsoberecoveredbyincubationingalactoseorlactose.
TechnicalSpecifications:
- Ligand: Erythrinacristagalli
- Matrix:4B-CL(crosslinkedagarose,4%)
- Particlesizerange:52–165µm
- Matrixactivation:Cyanogenbromide
- Matrixattachment:Amino
- Liganddensity:3mgECL/mldrainedgel
- Bindingcapacity:2.5mgasialofetuin/mldrainedgel
- Inhibiting/ElutingSugar:200mMLactose
- CarbohydrateforElution:.2MLactose
- BloodGroupSpecificity:T-antigen
- Form:50:50LiquidSUSPension,PBS(1x)pH7.4w/0.01%Thimerosal
- pHstABIlity:4–9
- Storagetemp:2–8°CDONOTFREEZE
- ApplicationNote:Calcium,Manganese,andZincarerequiredforbinding.
AdditionalInformation
| Product# | E-3345 |
|---|---|
| Appearance | LiquidSuspension |
| StorageTemp | 4°C |
