DTT; Cleland's Reagent

C-1029

3483-12-3

(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol

C₄H₁₀O₂S₂

154.25 Da

White crystalline powder

≥99.0%

10% solution in water. Clear and colorless

42-43°C

N/A

+4°C, under inert gas, desiccated

Not Applicable

DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. However, even DTT cannot reduce buried (solvent-inaccessible) disulfide bonds, so reduction of disulfide bonds is sometimes carried out under denaturing conditions (e.g., at high temperatures, or in the presence of a strong denaturant such as 6 M guanidinium chloride, 8 M urea, or 1% sodium dodecylsulfate). DTT is oftentimes used along with sodium dodecylsulfate in SDS-PAGE to further denature proteins by reducing their disulfide bonds to allow for better separation of proteins during electrophoresis. Because of the ability to reduce disulfide bonds, DTT can be used to denature CD38 on red blood cells. Conversely, the solvent exposure of different disulfide bonds can be assayed by their rate of reduction in the presence of DTT. DTT can also be used as an oxidizing agent. Its principal advantage is that effectively no mixed-disulfide species are populated, in contrast to other agents such as glutathione. In very rare cases, a DTT adduct may be formed, i.e., the two sulfur atoms of DTT may form disulfide bonds to different sulfur atoms; in such cases, DTT cannot cyclize since it has no such remaining free thiols.

12.3441.1996

MFCD00004877

X1009950-2

InChI=1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

C([C@H]([C@@H](CS)O)O)S

EK1610000

UN 3335

III

Hygroscopic. Protect from moisture. Protect from light.The pharmacological and toxicological properties of this product have not been fully investigated. Use caution when handling. Do not use this compound if you are not fully trained or are unaware of the hazards involved.

C-1029, DTT, SDS, diamond format.pdf

C-1029, J1220.pdf

C-1029, H1422B.pdf

C-1029, H1422A.pdf