Applications:
Blue-Separopore®4B-CL isCibacron™BlueF3G-AcovalentlyattachedtotheSeparopore®4B-CLmatrixbythetriazinecouplingmethod.Thestructureofthebluedyemakesitaveryversatiletoolforseparatingmanyproteins,e.g.albumin,interferon,lipoproteinsandbloodcoagulationfactors.Italsobindsseveralenzymesincludingkinases,dehydrogenases,andmostenzymesrequiringadenyl-containingcofactorse.g.NAD+.Thehighlycross-linkedmatrixprovidesastable,rigidmedium.
Blue-Separopore®4B-CL isadye-ligandaffinitymatrixforpurificationofalbumin,enzymes(includingNAD+ andNADP+),coagulation factors,interferonsandrelatedproteins.Theligand,CibacronBlueF3G-A,iscovalentlycoupledtoSeparopore®4B-CLviachlorotriazinering.Duetoabroadspectrumoftheaffinity,thisproductcanbeusedasageneralpurposeproteinpurification tooloftenusedfortheremovalofBSAfromserumfortheenrichmentoflessabundantproteins.
Technicalspecifications:
- Ligand:CibacronBlueF3G-A
- Matrix:Separopore®4B-CL(crosslinkedagarosebeads,4%)
- Particlesizerange:52–165µm
- Matrixactivation:Triazinecoupling
- Liganddensity:2–6µmolCibacronblueF3G-A/mldrainedgel
- Bindingcapacity: ~15mgbovineserumalbumin/mldrainedgel
- pHstABIlity:3–13
- Storage:2–8°C
- Suppliedas50%slurryin0.5MNaClcontaining0.02%thimerosal
AdditionalInformation
| Product# | B-3397 |
|---|
