AG Scientific/Biotin (Agarose) 6B-CL/B-3393/10 ml
商品编号：
B-3393-10ml
品牌：
agscientific
市场价：
¥12271.60
美元价：
7362.96
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Applications:
Biotin-Separopore®bindswithhighaffinitytoavidinandstreptavidinandisusedtopurify(strept)avidinlinkedbiomoleculessuchasproteinscoupledtostrept(avidin).Theinteractionofbiotinwithavidinisthestrongestknownnon-covalentinteractionbetweenaproteinanditsligand(Ka =1015 M-1).BondformationisrapidandisunaffectedbymostextremesofpH,organicsolventsandotherdenaturingagents.Immobilizedbiotinwillreleaseavidinifexposedto8Mguanidine-HClatpH1.5orifboiledinreducingSDS-PAGEsampleBuffer.Theseelutionconditionspermanentlydenaturetheavidinprotein. IthasbeenwidelyusedinbiochemistryandmolecularBIOLOGyfordetectingandlabelingproteins. Formildconditions,use2-Iminobiotin-Separopore®4B-CL(SKU#20181056).
Technicalspecifications:
- Ligand:Biotin
- Matrix:Separopore®4B-CL(crosslinkedagarosebeads,4%)
- Particlesizerange:52–165µm
- Molecularweightrange:6x104 –2x107
- Matrixactivation:Epoxy
- Matrixattachment:Carboxy(amidelinkagethroughdiaminopropanespacer);matrixcontainsresidualaminogroupswhicharechargedatphysiologicalpH
- Matrixspacer:13atoms
- Liganddensity:2–6 μmol/mldrainedgel
- Bindingcapacity:20–30 mgofavidin/mldrainedgel
- pHstABIlity:4–9
- Flowspecifications:70–140cm/h
- SuppliedassUSPensioninphosphatebufferedsalinecontaining0.02%sodiumazide
AdditionalInformation
| Product# | B-3393 |
|---|---|
| Appearance | LiquidSuspension |
| StorageTemp | 4°C |
