Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)RLGFLGSNTPHENH,correspondingtoaminoacidresidues2683-2696ofratIP3R2(Accession P29995). Intracellular,C-terminus.
- Westernblotanalysisofratbrainmembrane(lanes1and3)andratbasophilicleukemia(RBL)celllinelysate(lanes2and4):1,2. Anti-IP3 Receptor-2 (ITPR2) Antibody(#ACC-116),(1:200).
3,4.Anti-IP3 Receptor-2(ITPR2)Antibody,preincubatedwiththenegativecontrolantigen.
- ExpressionofIP3R2inratspinalcordImmunohistochemicalstainingofIP3R2inratspinalcordsectionsusing Anti-IP3 Receptor-2(ITPR2)Antibody(#ACC-116). A.IP3R2immunoreactivity(red)appearsinneuronalsoma(horizontalarrows)andprocesses(verticalarrows).B.NuclearstainingusingDAPIasthecounterstain(blue).C.MergedimageofAandB.
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Inositol1,4,5-trisphosphatereceptors(IP3R)areintracellularCa2+ releasechannelslocatedontheendoplasmicreticulum(ER)andmediateCa2+ mobilizationfromtheERtothecytoplasminresponsetothebindingofthesecondmessenger,inositol1,4,5-trisphosphate(IP3)1.IP3-inducedCa2+ releaseistriggeredbyvariousexternalstimuli,andmostnon-excitablecellsusethismechanismastheprimaryCa2+ signalingpathway.IP3Rsarethereforethoughttohaveimportantphysiologicalrolesinvariouscelltypesandtissues2.
ThreesubtypesofIP3Rs,derivedfromthreedistinctgenes,havebeenidentifiedinmammals3.AllthreereceptorshavesixtransmembranedomainsandaporeregionbetweenTM5andTM6.TheN-terminusaswellastheC-terminusarecytoplasmic.EachIP3RconsistsofanN-terminalligandbindingdomain(LBD)andaC-terminaldomainwhichislinkedbyalongregulatorydomain.TheC-terminusisconstitutivelyactive,suggestingthattheregulatorydomainisrequiredtomaintainthesuppressionofchannelactivity4.
Type2IP3R(IP3R2)isexpressedinvarioustissuesandcelllines.IP3R2mRNAislocalizedintheintralobularductcellsofthesuBMAndibulargland,theurinarytubulecellsofthekidney,theepithelialcellsofepididymalductsandthefolliculargranulosacellsoftheovary5.IP3R2isactiveduringosteoclastdifferentiation,anditsabsencecausesapartialdefectinosteoclasticdifferentiation.
Anti-IP3Receptor-2(ITPR2)Antibody(#ACC-116)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibody canbeusedinwesternblotandimmunohistochemistryapplications.IthasbeendesignedtorecognizeIP3R2fromrat,mouseandhumansamples.