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AG Scientific/Arachis hypogaea (Peanut) lectin (PNA) -Separopore®/A-2923/25 ml
Applications:
Arachishypogaea (PeanutAgglutinin,PNA) 4B-CLisusedforthepurificationofO-linkedglycoproteins.
Peanutagglutininisa110,000molecularweightlectincomposedoffouridenticalsubunitsofapproximately27,000daltonseach.PNAbindspreferentiallytoacommonlyoccurringstructure,galactosyl(β-1,3)N-acetylgalactosamine.Thiscarbohydratesequence(calledthe“T-antigen”)ispresentinmanyglycoconjugatessuchasMandNbloodgroups,gangliosides,andmanyothersolubleandmembrane-associatedglycoproteinsandglycolipids.PNAisusefulindistinguishingbetweennormalandtumortissuesandinassessingmalignancyintransitionalmucosa.Inaddition,PNAbindinghasbeenemployedasameasureofcellularmaturityinlymphoidtissues,todistinguishavarietyoflymphocytehumansubpopulationsandanimals,andtomeasurethelevelsoflymphoidcellpopulationsinmanydiseases.PresenceofCa++ canenhancePNAbinding.
TechnicalSpecifications:
- Ligandsource: Arachishypogaea(peanutLectin).
- Matrix:Separopore®4B-CL(crosslinkedagarosebeads,4%)
- Particlesizerange:52–165µm
- Matrixactivation:Cyanogenbromide
- Matrixattachment:Amino
- Liganddensity:5mgPeanutlectin/mldrainedgel.
- BindingCapacity:0.5–1.5mgofasialofetuin/mlofdrainedmedium.
- Inhibiting/ElutingSugar:200mMgalactose.
- CarbohydrateforElution:0.1Mlactoseor0.2Mgalactoseinbuffer.10-50mMN-acetylgalactosaminemayalsobeused.
- BloodGroupSpecificity:T-antigen
- Form:50:50LiquidSUSPension,PBS(1x)pH7.4w/0.01%Thimerosal
- pHstABIlity:4–9
- Applicationnote:Calciumisrequiredforbinding.Ca2+ concentrationshouldnotexceed0.5mMinthebuffer(tonotformawhiteprecipitate).
AdditionalInformation
| Product# | A-2923 |
|---|---|
| Appearance | LiquidSuspension |
| StorageTemp | 4°C |
