Lectinsareproteinsorglycoproteinsofnon-immuneoriginthatagglutinatecellsand/orprecipitatecomplexcarbohydrates.Lectinsarecapableofbindingglycoproteinseveninpresenceofvariousdetergents.ArachishypogaealectinorPeanutAgglutinin(PNA)isisolatedfrompeanutsandpurifiedbyaffinitychromatography.Thelectinhasamolecularweightof110kDaandconsistsoffouridenticalsubunitsofapproximately27kDaeach.PNAdoesnotagglutinatenormalhumanerythrocytes,butstronglyagglutinatesneuraminidasetreatederythrocytes.LectinPNAisspecificforterminalβ-galactoseandbindspreferentiallytoacommonlyoccurringstructure,galactosyl(β-1,3)N-acetylgalactosamine.PNAhaspotentanti-Tactivitysimilartotheanti-Tantibodyinhumansera.PNAhasbeenusedintumourtissuedeterminationfortransitionalmucosamalignancies.Thelectinalsoagglutinatesneuraminidase-treatedhumanerythrocytesat<0.1 µg/ml="" after="" trypsin="" treatment="" of="" cells="" and="" its="" activity="" is="" inhibited="" by="" lactose="" and="" galactose.="" though="" pna="" does="" not="" require="" any="" divalent="" cations="" for="" activity,="" the="" presence="" of="" calcium="" ions="" in="" diluents="" can="" enhance="" the="" binding="" of="" pna="" to="" receptors,="" possibly="" by="" neutralizing="" the="" negative="" charges="" on="" sialic="" acid="" residues="" adjacent="" to="" the="" receptor="" sequence.="" pna="" is="" useful="" in="" distinguishing="" between="" normal="" and="" tumor="" tissues="" and="" in="" assessing="" malignancy="" in="" transitional="" mucosa.="" in="" addition,="" pna="" binding="" can="" be="" used="" to="" measure="" cellular="" maturity="" in="" lymphoid="" tissues,="" to="" distinguish="" a="" variety="" of="" lymphocyte="" subpopulations="" in="" man="" and="" experimental="" animals,="" and="" to="" measure="" the="" levels="" of="" lymphoid="" cell="" populations="" in="" many="" diseases.="" pna="" can="" be="" employed="" in="" the="" fractionation="" of="" stem="" cells="" in="" mice="" for="" use="" in="" bone="" marrow="" transplantation="" across="" histocompatibility="" barriers.="" pna="" is="" silica-coated="" super="" paramagnetic="" beads="" covalently="" linked="" to="" peanut="" agglutinin="" (pna).="" peanut="" agglutinin="" is="" a="" non-glycosylated="" lectin="" with="" a="" high="" binding="" affinity="" for="" the="" tumor="" associated="" thomsen-friedenreich="" antigen="" (“t`-antigen)="" and="" binds="" preferentially="" to="" galactosyl="" (α-1,3)="" n-acetylgalactosamine.="" presence="" of="" ca2+="" can="" enhance="" pna="" binding.="">

AdditionalInformation

AdditionalInformation

Product#	A-2909
Appearance	LiquidSUSPension
Purity	HighPurityGrade
StorageTemp	4°C
Use	PNAcanbeusedindetectionofrelocalizationofTagantigeninlargebowelcarcinomaPNAcanbeusedtodistinguishbetweenhumanlymphocytesubsets.PNAcanbeusedasprobeinhistochemistryandimmunohistochemistryPNAcanbeusedinhumanerythrocyte/lymphocytestudiesToinvestigatethechangesrelatedtocelldifferentiation.Agglutinatesneuraminidase-treatedhumanerythrocytes.PNAisusefulindistinguishingbetweennormalandtumortissuesandinassessingmalignancyintransitionalmucosa.Tomeasurethelevelsoflymphoidcellpopulationsinmanydiseases.