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AG Scientific/Arachis hypogaea (Peanut) lectin (PNA) - Rhodamine (TRITC)/A-2901/2 mg
Lectinsareproteinsorglycoproteinsofnon-immuneoriginthatagglutinatecellsand/orprecipitatecomplexcarbohydrates. Lectinsarecapableofbindingglycoproteinseveninpresenceofvariousdetergents.
Arachishypogaea lectinorPeanutAgglutinin(PNA)isisolatedfrompeanutsandpurifiedbyaffinitychromatography.Thelectinhasamolecularweightof110kDaandconsistsoffouridenticalsubunitsofapproximately27kDaeach. PNAdoesnotagglutinatenormalhumanerythrocytes,butstronglyagglutinatesneuraminidasetreatederythrocytes.LectinPNAisspecificforterminalβ-galactoseandbindspreferentiallytoacommonlyoccurringstructure,galactosyl(β-1,3)N-acetylgalactosamine.PNAhaspotentanti-Tactivitysimilartotheanti-Tantibodyinhumansera. PNAhasbeenusedintumourtissuedeterminationfortransitionalmucosamalignancies.Thelectinalsoagglutinatesneuraminidase-treatedhumanerythrocytesat<0.1µg/mlaftertrypsintreatmentofcellsanditsactivityisinhibitedbylactoseandgalactose. ThoughPNAdoesnotrequireanydivalentcationsforactivity,thepresenceofcalciumionsindiluentscanenhancethebindingofPNAtoreceptors,possIBLybyneutralizingthenegativechargesonsialicacidresiduesadjacenttothereceptorsequence.
PNAisusefulindistinguishingbetweennormalandtumortissuesandinassessingmalignancyintransitionalmucosa.Inaddition,PNAbindingcanbeusedtomeasurecellularmaturityinlymphoidtissues,todistinguishavarietyoflymphocytesubpopulationsinmanandexperimentalanimals,andtomeasurethelevelsoflymphoidcellpopulationsinmanydiseases.PNAcanbeemployedinthefractionationofstemcellsinmiceforuseinbonemarrowtransplantationacrosshistocompatibilitybarriers.
Rhodamine(TRITC)labeledPNAhasanappropriatenumberofFluorochromesboundtoprovidetheoptimumstainingcharacteristicsforthislectin.Thisconjugateissuppliedessentiallyfreeofunconjugatedfluorochromes.Theexcitationmaximumisat550nmandtheemissionmaximumisat575nm.
TechnicalSpecifications:
- LigandSource: Arachishypogaea
- MolecularWeight: 110kDa
- CarbohydrateSpecificity: Galβ1,3GalNAc(T-antigen)
- Inhibiting/ElutingSugar:Galactose
- CarbohydrateforElution: 0.2MGalactose
- BloodGroupSpecificity: Tantigen(M,N)
- Conjugate:Rhodamine
- Form: Liquid
- Isoelectric(pI): 5.5-6.5
AdditionalInformation
| Synonyms | PeanutAgglutinin;PNA;Galactose-bindingLectin |
|---|---|
| Product# | A-2901 |
| Appearance | Liquid |
| Purity | HighPurityGrade |
| StorageTemp | -20°C |
| Use | BiotynylatedPNAcanbeusedforthedetectionofrelocalizationofTagantigeninlargebowelcarcinomABIotynylatedPNAcanbeusedtodistinguishbetweenhumanlymphocytesubsets.BiotynylatedPNAcanbeusedasprobeinhistochemistryandimmuno-histochemistry.BiotynylatedPNAcanbeusedinhumanerythrocyte/lymphocytestudies. |
