Lectinsareproteinsorglycoproteinsofnon-immuneoriginthatagglutinatecellsand/orprecipitatecomplexcarbohydrates. Lectinsarecapableofbindingglycoproteinseveninpresenceofvariousdetergents.
Arachishypogaea lectinorPeanutAgglutinin(PNA)isisolatedfrompeanutsandpurifiedbyaffinitychromatography.Thelectinhasamolecularweightof110kDaandconsistsoffouridenticalsubunitsofapproximately27kDaeach. PNAdoesnotagglutinatenormalhumanerythrocytes,butstronglyagglutinatesneuraminidasetreatederythrocytes.LectinPNAisspecificforterminalβ-galactoseandbindspreferentiallytoacommonlyoccurringstructure,galactosyl(β-1,3)N-acetylgalactosamine.PNAhaspotentanti-Tactivitysimilartotheanti-Tantibodyinhumansera. PNAhasbeenusedintumourtissuedeterminationfortransitionalmucosamalignancies.Thelectinalsoagglutinatesneuraminidase-treatedhumanerythrocytesat<0.1µg/mlaftertrypsintreatmentofcellsanditsactivityisinhibitedbylactoseandgalactose. ThoughPNAdoesnotrequireanydivalentcationsforactivity,thepresenceofcalciumionsindiluentscanenhancethebindingofPNAtoreceptors,possIBLybyneutralizingthenegativechargesonsialicacidresiduesadjacenttothereceptorsequence.
PNAisusefulindistinguishingbetweennormalandtumortissuesandinassessingmalignancyintransitionalmucosa.Inaddition,PNAbindingcanbeusedtomeasurecellularmaturityinlymphoidtissues,todistinguishavarietyoflymphocytesubpopulationsinmanandexperimentalanimals,andtomeasurethelevelsoflymphoidcellpopulationsinmanydiseases.PNAcanbeemployedinthefractionationofstemcellsinmiceforuseinbonemarrowtransplantationacrosshistocompatibilitybarriers.
FluoresceinlabeledPeanutagglutininhasanappropriatenumberofFluorochromesboundtoprovidetheoptimumstainingcharacteristicsforthislectin.Thisconjugateissuppliedessentiallyfreeofunconjugatedfluorochromes. FITC-conjugatedPNAhasanexcitationat495nmandanemissionatabout515nm. FluorescentConjugatesare extremely lightsensitive.
TechnicalSpecifications:
- LigandSource: Arachishypogaea
- MolecularWeight: 110kDa
- CarbohydrateSpecificity: Galβ1,3GalNAc(T-antigen)
- Inhibiting/ElutingSugar:Galactose
- CarbohydrateforElution: 0.2MGalactose
- BloodGroupSpecificity: Tantigen(M,N)
- Conjugate:FITC
- Form: Liquid
- Isoelectric(pI): 5.5-6.5
AdditionalInformation
Synonyms | PeanutAgglutinin;PNA;Galactose-bindingLectin |
---|---|
Product# | A-2897 |
Appearance | Liquid |
Purity | HighPurityGrade |
StorageTemp | -20°C |
Use | BiotynylatedPNAcanbeusedforthedetectionofrelocalizationofTagantigeninlargebowelcarcinomABIotynylatedPNAcanbeusedtodistinguishbetweenhumanlymphocytesubsets.BiotynylatedPNAcanbeusedasprobeinhistochemistryandimmuno-histochemistry.BiotynylatedPNAcanbeusedinhumanerythrocyte/lymphocytestudies. |