Lectinsareproteinsorglycoproteinsofnon-immuneoriginthatagglutinatecellsand/orprecipitatecomplexcarbohydrates. Lectinsarecapableofbindingglycoproteinseveninpresenceofvariousdetergents.
Arachishypogaea lectinorPeanutAgglutinin(PNA)isisolatedfrompeanutsandpurifiedbyaffinitychromatography.Thelectinhasamolecularweightof110kDaandconsistsoffouridenticalsubunitsofapproximately27kDaeach. PNAdoesnotagglutinatenormalhumanerythrocytes,butstronglyagglutinatesneuraminidasetreatederythrocytes.LectinPNAisspecificforterminalβ-galactoseandbindspreferentiallytoacommonlyoccurringstructure,galactosyl(β-1,3)N-acetylgalactosamine.PNAhaspotentanti-Tactivitysimilartotheanti-Tantibodyinhumansera. PNAhasbeenusedintumourtissuedeterminationfortransitionalmucosamalignancies.Thelectinalsoagglutinatesneuraminidase-treatedhumanerythrocytesat<0.1µg/mlaftertrypsintreatmentofcellsanditsactivityisinhibitedbylactoseandgalactose. ThoughPNAdoesnotrequireanydivalentcationsforactivity,thepresenceofcalciumionsindiluentscanenhancethebindingofPNAtoreceptors,possIBLybyneutralizingthenegativechargesonsialicacidresiduesadjacenttothereceptorsequence.
PNAisusefulindistinguishingbetweennormalandtumortissuesandinassessingmalignancyintransitionalmucosa.Inaddition,PNAbindingcanbeusedtomeasurecellularmaturityinlymphoidtissues,todistinguishavarietyoflymphocytesubpopulationsinmanandexperimentalanimals,andtomeasurethelevelsoflymphoidcellpopulationsinmanydiseases.PNAcanbeemployedinthefractionationofstemcellsinmiceforuseinbonemarrowtransplantationacrosshistocompatibilitybarriers.
TechnicalSpecifications:
- LigandSource: Arachishypogaea
- Molecularweight:25.2kDa(monomer)and100.8kDa(nativetetramer)-basedontheaminoacidsequence
- Carbohydratespecificity: Gal3GalNAc,Galβ1
- InhibitingSugar/ElutingSugar:Galactose
- CarbohydrateforElution:200mMGalactose
- Bloodgroupspecificity: Tantigen(M,N)
- Form:Lyophilized
- Isoelectric(pI):5.5-6.5
AdditionalInformation
| Synonyms | Peanutagglutinin;PNA;Galactose-bindinglectin |
|---|---|
| Product# | A-2895 |
| Appearance | Liquid |
| StorageTemp | -20°C |
| Use | PNAcanbeusedindetectionofrelocalizationofTagantigeninlargebowelcarcinomaPNAcanbeusedtodistinguishbetweenhumanlymphocytesubsets.PNAcanbeusedasprobeinhistochemistryandimmunohistochemistryPNAcanbeusedinhumanerythrocyte/lymphocytestudies |
