Blasticidin S; Bla-S

B-1247

3513-03-9

(2S,3S,6R)-3-[[(3S)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride

C₁₇H₂₆N₈O₅•HCl

458.9 Da

White powder

≥98.0%

Soluble in water. Clear and faint yellow solution at 5 mg/ml in water. Insoluble in organic solvents.

218.0-225.0 °C

+4°C, desiccated

Antibacterial

Blasticidin is an efficient selection antibiotic that acts on both eukaryotic and prokaryotic cells. Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery. In biological research, specifically genetic engineering, it is used to select transformed cells which have been engineered to carry a resistance gene for blasticidin. In short, DNA of interest is fused to DNA encoding a resistance gene, and then is transformed into cells. After allowing time for recovery and for cells to begin transcribing and translating their new DNA, blasticidin is added. Now only the cells that have the new DNA can grow. Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase. Resistance to blasticidin is also conferred by the blastcidin S acetyltransferase gene (bls) from Streptoverticillum sp, and the blasticidin S deaminase gene (BSD) from Apergillus terreus. Cell death induced by blasticidin occurs rapidly, allowing for selection of transfected cell lines carrying a blasticidin resistance gene within one week.

MFCD02091640

X1339381-2

InChI=1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H

[H]/N=C(N)/N(C)CCC(CC(=O)NC1C=CC(OC1C(=O)O)n2ccc(nc2=O)N)N.Cl

Not Applicable

Not Applicable

UN 2811

II

Not Applicable

Avoid contact with skin, eyes or clothing. Use personal protective equipment as required.Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray.Do not eat, drink or smoke when using this product. Corrosive hazard. Wear protectivegloves/clothing and eye/face protection. Thermal decomposition can lead to release oftoxic/corrosive gases and vapors.

Yes

VIRUS-CELL INTERACTIONS:Human Apolipoprotein E Is Required for Infectivity and Production of Hepatitis C Virus in Cell CultureKyung-Soo Chang, Jieyun Jiang, Zhaohui Cai, and Guangxiang LuoJ. Virol., Dec 2007; 81: 13783 - 13793.

http://jvi.asm.org/cgi/reprint/81/24/13783

B-1247, Blasticidin S HCl, SDS, diamond format.pdf

B-1247, J1268.pdf

B-1247, H1272.pdf

B-1247, H1252A.pdf