5-Bromo-4-chloro-3-indolylβ-D-galactopyranosideismostcommonlyknownas X-Gal.X-galisahistochemicalsubstrateforβ-galactosidasewhichcleavesX-Galtoyieldaninsolubleblueprecipitate.
X-GalisamainstayinmolecularBIOLOGycloningapplicationsinwhichitisusedtodetecttheactivityofβ-galactosidase.X-GalisusedtodetecttheinsertionofforeignDNAintothelacZregionofaplasmidDNA.InsertionofDNAintothelacZregionresultsinthelossofβ-galactosidaseactivity.
X-galisusedtoindicatewhetheracellexpressestheβ-glactosidaseenzyme,whichisencodedbythelacZgene,inatechniquecalledbluewhitescreening.Bacterialcellsthatretainactiveβ-galactosidasewillresultincharacteristicbluecolonies.Successfuldisruptionofthe lacZα genedisruptstheα-complementationoftheβ-galactosidasegeneandtheprecipitatedoesnotform,resultinginwhitecolonies.
AdditionalInformation
| Synonyms | BCIG |
|---|---|
| Product# | X-1015 |
| CAS# | 7240-90-6 |
| ChemicalName | 5-Bromo-4-Chloro-3-Indolyl-B-D-Galactopyranoside |
| Formula | C14H15BrClNO6 |
| MW | 408.61 |
| Appearance | WhitePowder |
| Purity | 99%(TLC) |
| Solubility | Soluble2%inDMF |
| MeltingPoint | 236-238°C |
| BoilingPoint | 698.00°Cat760mmHg |
| StorageTemp | +4°C |
| Use | Achromogenichistochemicalsubstrateforβ-galactosidaseusedtodetectDNAinsertionsintothelacZregionofplasmidDNA |
| MDLNumber | MFCD00005666 |
|---|---|
| ChemACX | X1009969-1 |
| InChI | InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1 |
| SMILES | c1cc(c(c2c1[nH]cc2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br |
