ThisbroadspectrumProteaseandPhosphataseInhibitorcocktailhasbeenspecificallydesignedtoprovidecompleteprotectionofvaluableproteinsofinterestfromdegradationduringextractionandpurification.
FeaturesandBenefits:Completeprotectionfromproteasesandphosphatasesinonesinglecocktail.
- AllourcocktailscontainAEBSF,thepreferredserineproteaseinhibitor,toprovidesuperiorprotectionwithunmatchedstABIlity,convenienceandreliability.
- ThecocktailformulationsareavailablewithorwithoutEDTAwhichmayinterferewithproteinstabilityorcertaindownstreamapplications.
- OurcocktailsarelyophilizedsolidswhichdissolveinstantaneouslymakingthemreADIlyavailablefortheirinstantaneousinhibitoryactionandareeasiertousethantabletsthatrequiresplitting,vortexingandtimeconsumingdissolution.
- Ourlyophilizedsolidsareguaranteedforstability,reproducibilityandhaveashelflifeofoverayear.Mostothercommerciallyavailablecocktailsmadeandsoldinaqueoussolutionsofferverylowguaranteeofstabilityafter1monthofbeingmanufactured(Litref:Bestatin,Leupeptinsolutioninwaterarestablefor1monthat-20oC).
- Economical:Morecosteffectivethanothercommerciallyavailableformulations.
- Reliable:Countonourexpertiseintheproductionofhighqualitycocktailsthathavebeensuccessfullyusedfor20+yearsbythousandsofresearchersworldwide.
ProductDescription:WhiteLyophilizedSolid.Thecocktailcontains16inhibitorstargetingagainstthemajorclassesofproteasesandphosphatasesasoutlinedinthefollowingtable
Products | TargetProteases | Products | TargetPhosphatases |
SerineProteases | Imidazole | AlkalinePhosphatases | |
BroadSpectrum,SerineProteases | SodiumOrthovanadate | ProteinTyrosinePhosphatase,AlkalinePhosphatase | |
AminopeptidaseBandLeucineAminopeptidase | SodiumMolyBDate | AcidandPhosphoproteinPhosphatases | |
CysteineProteases | NaF | AcidPhosphatases | |
CysteineProteasesandTrypsin-likeProteases | SodiumPyrophosphate | PP1andPP2A | |
AsparticProteases | Glycerophosphate | Ser/ThrPhosphatases | |
Metalloproteases Metalloendopeptidases | NaTartrateDihydrate | AcidPhosphatases | |
Papain,CathepsinA/B | Bromotetramisoleoxalate |
|
RecommendedUsage:Thiscocktailhasbeenoptimizedforeffectiveness,convenience,stability,reproducibilityandhasbeentestedforgeneraluse.Itinhibitsactivityinextractsfromalmostanytissueorcelltypesincludinganimals,plants,yeasts,bacteriaandfungi.
Reconstituteeachvialwith1mlofwatertoobtainaconcentratedstocksolution.Dilutethisconcstocksolutionto1Xbeforeuse.Recommendeddilutionis1:100(basedontheapplication).Onevialisrecommendedfor10g-20goftissueorcellextract.
PerformanceActivityAssay: ComparisonofourTurbochargedProteaseandPhosphataseInhibitorCocktailswithCompetitor’scommercialtablets.
Figure1: Usingageneralproteaseactivityassayandpancreasextract,ourTurbochargedCocktailsweretestedagainstCompetitor’scommerciallyavailabletabletsunderthesameconditions.Theresultswerebasedonproteaseactivityintheabsenceofanyinhibitorcocktails(Test1);comparedtoproteaseactivityinthepresenceofCompetitorsProteaseInhibitorTablets(Test2);TurbochargedProteaseInhibitorCocktail(T-2492/Test3);TurbochargedProtease+PhosphataseInhibitorCocktail(T-2495/Test4);andBlank
Figure2: Usingageneralphosphataseactivityassaythealkalinephosphataseactivitywasdeterminedwithnoinhibitorcocktailadded(Test1);comparedtoPhosphataseactivityinthepresenceofTurbochargedPhosphataseInhibitorCocktail(T-2494/Test2);TurbochargedProtease+PhosphataseInhibitorCocktail(T-2495/Test3);andBlank
TheresultsindicatethatourTurbochargedCocktailsshowedbetterperformance(>97%inhibition)thantheCompetitorsproductwithourTurbochargedProtease+PhosphataseinhibitorCocktailsbeingtheonlyoneofitskindavailableinthemarkettodayinhibitingbothProteases+Phosphatasesavailableasalyophilizedsolidinasinglevial.
AdditionalInformation
| Product# | T-2495 |
|---|---|
| Appearance | LyophilizedSolid.Hygroscopic |
| Purity | SuitableforuseasProteaseandPhosphataseInhibitors |
| Solubility | Solubleinwater,aqueousbuffersoradddirectlytoextractionmedia |
| StorageTemp | -20ºC |
