ThisbroadspectrumPhosphataseInhibitorcocktailhasbeenspecificallydesignedtoprovidecompleteprotectionofvaluableproteinsofinterestfromdegradationduringextractionandpurification.
FeaturesandBenefits:Completeprotectionfromphosphatasesinonesinglecocktail.
- OurcocktailsarelyophilizedsolidswhichdissolveinstantaneouslymakingthemreADIlyavailablefortheirinstantaneousinhibitoryactionandareeasiertousethantabletsthatrequiresplitting,vortexingandtimeconsumingdissolution.
- OurlyophilizedsolidsareguaranteedforstABIlity,reproducibilityandhaveashelflifeofoverayear.Mostothercommerciallyavailablecocktailsmadeandsoldinaqueoussolutionsofferverylowguaranteeofstabilityafter1monthofbeingmanufactured.
- Economical:Morecosteffectivethanothercommerciallyavailableformulations.
- Reliable:Countonourexpertiseintheproductionofhighqualitycocktailsthathavebeensuccessfullyusedfor20+yearsbythousandsofresearchersworldwide.
ProductDescription:WhiteLyophilizedSolid.Thecocktailcontains8inhibitorstargetingagainstthemajorclassesofphosphatasesasoutlinedinthefollowingtable
Products | TargetPhosphatases | Products | TargetPhosphatases |
Imidazole | AlkalinePhosphatases | SodiumPyrophosphate | PP1andPP2A |
SodiumOrthovanadate | ProteinTyrosinePhosphatase,AlkalinePhosphatase | Glycerophosphate | Ser/ThrPhosphatases |
SodiumMolyBDate | AcidandPhosphoproteinPhosphatases | NaTartrateDihydrate | AcidPhosphatases |
NaF | AcidPhosphatases | Bromotetramisoleoxalate | AlkalinePhosphatases |
RecommendedUsage:Thiscocktailhasbeenoptimizedforeffectiveness,convenience,stability,reproducibilityandhasbeentestedforgeneraluse.Itinhibitsactivityinextractsfromalmostanytissueorcelltypesincludinganimals,plants,yeasts,bacteriaandfungi.
Reconstituteeachvialwith1mlofwatertoobtainaconcentratedstocksolution.Dilutethisconcstocksolutionto1Xbeforeuse.Recommendeddilutionis1:100(basedontheapplication).Onevialisrecommendedfor10g-20goftissueorcellextract.
PerformanceActivityAssay: ComparisonofourTurbochargedProteaseandPhosphataseInhibitorCocktailswithCompetitor’scommercialtablets.
Figure1: Usingageneralproteaseactivityassayandpancreasextract,ourTurbochargedCocktailsweretestedagainstCompetitor’scommerciallyavailabletabletsunderthesameconditions.Theresultswerebasedonproteaseactivityintheabsenceofanyinhibitorcocktails(Test1);comparedtoproteaseactivityinthepresenceofCompetitorsProteaseInhibitorTablets(Test2);TurbochargedProteaseInhibitorCocktail(T-2492/Test3);TurbochargedProtease+PhosphataseInhibitorCocktail(T-2495/Test4);andBlank
Figure2: Usingageneralphosphataseactivityassaythealkalinephosphataseactivitywasdeterminedwithnoinhibitorcocktailadded(Test1);comparedtoPhosphataseactivityinthepresenceofTurbochargedPhosphataseInhibitorCocktail(T-2494/Test2);TurbochargedProtease+PhosphataseInhibitorCocktail(T-2495/Test3);andBlank
TheresultsindicatethatourTurbochargedCocktailsshowedbetterperformance(>97%inhibition)thantheCompetitorsproductwithourTurbochargedProtease+PhosphataseinhibitorCocktailsbeingtheonlyoneofitskindavailableinthemarkettodayinhibitingbothProteases+Phosphatasesavailableasalyophilizedsolidinasinglevial.
AdditionalInformation
| Product# | T-2494 |
|---|---|
| Appearance | WhiteLyophilizedSolid.Hygroscopic |
| Purity | SuitableforuseasPhosphataseInhibitors |
| Solubility | Solubleinwater,aqueousbuffersoradddirectlytoextractionmedia |
| StorageTemp | -20ºC |
