G-418sulfate,alsoknownasgeneticin,isanaminoglycosideantibioticsimilarinstructuretogentamicinB1. G418sulfate(Geneticin)isusedroutinelyinmoleculargeneticsexperimentsforselectingeukaryotictransformants.ItiscommonlyusedinplantandmammaliancellcultureasaselectableMarker.G418sulfateisfreelysolubleinwater.Bacteriaandalgaerequire5µg/mlorlesswhileanimalcellsmayrequire300-500µg/ml. Itfunctionsbycausingachainofnonsensemutationsduringtranslation,ultimatelyresultingininhibitionofpolypeptidesynthesisandproteinelongation.ResistancetoG-418isconferredthroughthenptll geneinplantcellsandtheneogeneinmammaliancells,whichencodeaminoglycoside3"-phosphotransferase.Forgeneselectionapplications,akillcurvecanbemadetodeterminetheminimumeffectiveG418concentrationtokillnon-resistantcells.
G418longstandingapplicationasageneselectionantibiotic hasapowerfulnewroleinGenomeeditingutilizingtheCRISPR/Cas9system.
AdditionalInformation
| Synonyms | Geneticin |
|---|---|
| Product# | G-1033 |
| CAS# | 108321-42-2 |
| Formula | C20H40O10N4.2H2SO4 |
| MW | 692.7 |
| Appearance | whitetooff-whitepowder |
| Purity | >700μg/mg |
| Solubility | SolubleinWaterandAqueousBuffers |
| StorageTemp | +4°C |
| Use | Anantibioticwithpotentantiparasiticandantiamoebicactivity. |
