BlasticidinS.Hclisanaminoacylnucleosideantibioticisolatedfrom Streptomycesgriseochromogenes.Itshowshighefficacyagainstbotheukaryoticandprokaryoticcells.Asidefromitsantimicrobialproperties,blasticidinhasdisplayedantiviral,antitumor,andantifungalpropertiesandhasbeenusedasaselectionagent.Ithasbeenusedtoselectforcellscontainingthe bls, bsr,or BSD resistancegeneaswellasHEK293-TcellsandHEK-D5cells.Additionally,blasticidiniseffectiveinpreventingcellculturecontaminationandstudyingproteinsynthesis.
BlasticidinS.Hclfunctionsbyinhibitingpeptidebondformationattheribosome.Itblockshydrolysisofpeptidyl-tRNAduetothelargeribosomalsubunit"sincreasedaffinityfortRNA. BlasticidinS.Hcl"s,longstandingapplicationasageneselectionantibiotic,hasapowerfulnewroleinGenomeeditingutilizingtheCRISPR/Cas9system.
AdditionalInformation
| Product# | B-1247-SOL |
|---|---|
| CAS# | 3513-03-9 |
| Formula | C17H26N8O5.HCl |
| MW | 458.9 |
| Appearance | Liquid,10mg/mlinHepesbuffer(100mgtotal) |
| Purity | ≥98.0% |
| Solubility | Clearandfaintyellowsolution(5mg/mlinwater) |
| StorageTemp | +4ºC |
| MerckIndex | 14,1316 |
|---|
